# Test file for ASLAssay
asl_assays:
  - id: "ASL:001"
    name: "Airway surface liquid height"
    has_specified_output:
      id: "ASL:001-output"
      name: "ASL:001 measurement results"
      asl_height_um:
        value: "7.2"
        unit:
          id: "UO:0000017"
          name: "micrometer"
      periciliary_layer_depth:
        value: "5.1"
        unit:
          id: "UO:0000017"
          name: "micrometer"
      mucus_layer_thickness:
        value: "35"
        unit:
          id: "UO:0000017"
          name: "micrometer"
      ion_composition: "Cl- 125 mM, Na+ 140 mM, K+ 15 mM"
      asl_ph:
        value: "7.1"
        unit:
          id: "UO:0000196"
          name: "pH"
    assay_date: "2024-02-10"
    informs_on_key_event:
      id: "KE:ke-decreased-asl"
      name: "Decreased ASL height"
      biological_action: decreased
      level_of_biological_organization: cellular
    study_subject:
      subject_type: CellularSystem
      id: "soma:culture-002"
      name: "Primary HBE ALI culture"
      cell_type:
        id: "CL:0002328"
        name: "bronchial epithelial cell"
      model_species:
        id: "NCBITaxon:9606"
        name: "Homo sapiens"
    follows_protocols:
      - protocol_type: ImagingProtocol
        id: "PROTOCOL:asl-001"
        name: "Confocal microscopy for ASL height"
        spatial_resolution:
          value: "1.5"
          unit:
            id: "UO:0000017"
            name: "micrometer"
        fluorescent_labeling: "Texas Red-dextran"
        temperature_control:
          value: "37"
          unit:
            id: "UO:0000027"
            name: "degree Celsius"