# Test file for CFTRFunctionAssay
cftr_assays:
  - id: "CFTR:001"
    name: "CFTR chloride secretion"
    has_specified_output:
      id: "CFTR:001-output"
      name: "CFTR:001 measurement results"
      cftr_chloride_secretion:
        value: "15.2"
        unit:
          id: "UO:0000274"
          name: "microampere per square centimeter"
      cftr_forskolin_response:
        value: "12.8"
        unit:
          id: "UO:0000274"
          name: "microampere per square centimeter"
      inhibitor_sensitive_current:
        value: "14.5"
        unit:
          id: "UO:0000274"
          name: "microampere per square centimeter"
      cftr_specific_current:
        value: "13.9"
        unit:
          id: "UO:0000274"
          name: "microampere per square centimeter"
      sweat_chloride_concentration:
        value: "25"
        unit:
          id: "UO:0000308"
          name: "milliequivalent per liter"
      nasal_potential_difference:
        value: "-18.5"
        unit:
          id: "UO:0000247"
          name: "millivolt"
    stimulation_agent: "forskolin 10 uM"
    inhibitor_used: "CFTRinh-172"
    assay_date: "2024-04-12"
    informs_on_key_event:
      id: "KE:ke-decreased-cftr"
      name: "Decreased CFTR function"
      biological_action: decreased
      level_of_biological_organization: molecular
    study_subject:
      subject_type: CellularSystem
      id: "soma:culture-006"
      name: "Primary HBE ALI culture"
      cell_type:
        id: "CL:0002328"
        name: "bronchial epithelial cell"
      model_species:
        id: "NCBITaxon:9606"
        name: "Homo sapiens"
    follows_protocols:
      - id: "PROTOCOL:cftr-001"
        name: "Ussing chamber short-circuit current protocol"
      - id: "PROTOCOL:cftr-prep-001"
        name: "Tissue mounting and equilibration protocol"
        description: "Pre-assay protocol for mounting tissue in Ussing chamber and equilibrating"