# Test file for GobletCellAssay
goblet_cell_assays:
  - id: "GCM:001"
    name: "MUC5AC expression and goblet cell quantification"
    has_specified_output:
      id: "GCM:001-output"
      name: "GCM:001 measurement results"
      muc5ac_mrna_expression:
        value: "4.8"
        unit:
          id: "UO:0000193"
          name: "fold change"
      goblet_cell_percentage:
        value: "28"
        unit:
          id: "UO:0000187"
          name: "percent"
      goblet_cell_count:
        value: "85"
        unit:
          id: "UO:0000189"
          name: "count"
      muc5ac_protein_expression:
        value: "3.2"
        unit:
          id: "UO:0000193"
          name: "fold change"
      muc5b_mrna_expression:
        value: "1.8"
        unit:
          id: "UO:0000193"
          name: "fold change"
      muc5b_protein_expression:
        value: "1.5"
        unit:
          id: "UO:0000193"
          name: "fold change"
      muc5ac_muc5b_ratio:
        value: "2.7"
        unit:
          id: "UO:0000190"
          name: "ratio"
      mucin_secretion_rate:
        value: "12.5"
        unit:
          id: "UO:0000298"
          name: "microgram per hour"
      percent_solids:
        value: "4.2"
        unit:
          id: "UO:0000187"
          name: "percent"
      goblet_to_ciliated_ratio:
        value: "0.45"
        unit:
          id: "UO:0000190"
          name: "ratio"
    assay_date: "2024-06-15"
    informs_on_key_event:
      id: "KE:ke2-goblet-hyperplasia"
      name: "Goblet cell hyperplasia"
      biological_action: increased
      level_of_biological_organization: cellular
    study_subject:
      subject_type: CellularSystem
      id: "soma:culture-004"
      name: "Primary HBE ALI culture"
      cell_type:
        id: "CL:0002328"
        name: "bronchial epithelial cell"
      model_species:
        id: "NCBITaxon:9606"
        name: "Homo sapiens"
    follows_protocols:
      - protocol_type: StainingProtocol
        id: "PROTOCOL:stain-001"
        name: "AB-PAS staining for goblet cells"
        staining_type: "AB-PAS"
        fixation_method: "4% paraformaldehyde"
        counterstain: "hematoxylin"