# Test file for MucociliaryClearanceAssay
mcc_assays:
  - id: "MCC:001"
    name: "Mucociliary transport rate"
    has_specified_output:
      id: "MCC:001-output"
      name: "MCC:001 measurement results"
      transport_rate:
        value: "45.3"
        unit:
          id: "UO:0000103"
          name: "micrometer per second"
      transport_directionality: normal
      mucus_layer_thickness:
        value: "28"
        unit:
          id: "UO:0000017"
          name: "micrometer"
      percentage_active_transport:
        value: "82"
        unit:
          id: "UO:0000187"
          name: "percent"
      particle_clearance_time:
        value: "120"
        unit:
          id: "UO:0000010"
          name: "second"
    assay_date: "2024-03-05"
    informs_on_key_event:
      id: "KE:ke3-impaired-mcc"
      name: "Impaired mucociliary clearance"
      biological_action: impaired
      level_of_biological_organization: tissue
    study_subject:
      subject_type: CellularSystem
      id: "soma:culture-007"
      name: "Primary HBE ALI culture"
      cell_type:
        id: "CL:0002328"
        name: "bronchial epithelial cell"
      model_species:
        id: "NCBITaxon:9606"
        name: "Homo sapiens"
    follows_protocols:
      - protocol_type: ImagingProtocol
        id: "PROTOCOL:mcc-001"
        name: "Fluorescent microsphere tracking for MCC"
        fluorescent_tracer: "1-um fluorescent microspheres"
        particle_tracking_method: "automated particle tracking"
        particle_size:
          value: "1"
          unit:
            id: "UO:0000017"
            name: "micrometer"
        imaging_frame_rate:
          value: "30"
          unit:
            id: "UO:0000105"
            name: "hertz"