Subchronic inhalation of coal dust particulate matter 10 induces bronchoalveolar hyperplasia and decreases MUC5AC expression in male Wistar rats.
Study subjects
Male Wistar rat, 14-day coal dust exposure
Male Wistar rats exposed to coal dust PM10 for 14 days; the abstract reports no age, weight or group size.
Male Wistar rat, 28-day coal dust exposure
Male Wistar rats exposed to coal dust PM10 for 28 days; the abstract reports no age, weight or group size.
Male Wistar rat, coal dust exposure, lung histology
Male Wistar rats exposed to coal dust PM10 for 14 or 28 days; the abstract reports no age, weight or group size.
Goblet cell assays
MUC5AC expression by CLSM - coal dust PM10, 14 days
MUC5AC expression in male Wistar rat lung measured by confocal laser scanning microscopy after 14 days of coal dust PM10 inhalation at 6.25, 12.5 and 25 mg/m3. The paper reports no significant difference in MUC5AC expression for the 14-day exposure (p>0.05). No measurement values, group sizes or exact p-values are given in the available abstract, so the output records below carry no measurement slots.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Coal dust PM10, 6.25 mg/m3, 14 days EXPOSURE:kania-coaldust-6p25mgm3-14d |
coal dust ENVO:02000099 |
6.25 milligram per cubic meter | 14 day |
| Coal dust PM10, 12.5 mg/m3, 14 days EXPOSURE:kania-coaldust-12p5mgm3-14d |
coal dust ENVO:02000099 |
12.5 milligram per cubic meter | 14 day |
| Coal dust PM10, 25 mg/m3, 14 days EXPOSURE:kania-coaldust-25mgm3-14d |
coal dust ENVO:02000099 |
25 milligram per cubic meter | 14 day |
Outputs
| Group | Output | Description |
|---|---|---|
| treated | MUC5AC, 6.25 mg/m3, 14 days GCM:kania-muc5ac-clsm-14d-output-6p25 · under EXPOSURE:kania-coaldust-6p25mgm3-14d |
|
| treated | MUC5AC, 12.5 mg/m3, 14 days GCM:kania-muc5ac-clsm-14d-output-12p5 · under EXPOSURE:kania-coaldust-12p5mgm3-14d |
|
| treated | MUC5AC, 25 mg/m3, 14 days GCM:kania-muc5ac-clsm-14d-output-25 · under EXPOSURE:kania-coaldust-25mgm3-14d |
Evidence
There was no significant difference in MUC5AC, EGF, and EGFR expressions for 14-d exposure (p>0.05).
This sentence is the paper's entire statistical result for MUC5AC at 14 days and is what the null finding recorded on this assay rests on.
EGFR and MUC5AC were measured by a confocal laser scanning microscope.
Names the measurement technique this assay records, matching the confocal microscopy protocol it follows.
MUC5AC expression by CLSM - coal dust PM10, 28 days
MUC5AC expression in male Wistar rat lung measured by confocal laser scanning microscopy after 28 days of coal dust PM10 inhalation at 6.25, 12.5 and 25 mg/m3. The paper reports a significant difference in MUC5AC expression for the 28-day exposure (p<0.05) and its conclusion states the direction is a decrease. Only the significance threshold is reported - no measurement values, group sizes or exact p-values are given in the available abstract.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Coal dust PM10, 6.25 mg/m3, 28 days EXPOSURE:kania-coaldust-6p25mgm3-28d |
coal dust ENVO:02000099 |
6.25 milligram per cubic meter | 28 day |
| Coal dust PM10, 12.5 mg/m3, 28 days EXPOSURE:kania-coaldust-12p5mgm3-28d |
coal dust ENVO:02000099 |
12.5 milligram per cubic meter | 28 day |
| Coal dust PM10, 25 mg/m3, 28 days EXPOSURE:kania-coaldust-25mgm3-28d |
coal dust ENVO:02000099 |
25 milligram per cubic meter | 28 day |
Outputs
| Group | Output | Description |
|---|---|---|
| treated | MUC5AC, 6.25 mg/m3, 28 days GCM:kania-muc5ac-clsm-28d-output-6p25 · under EXPOSURE:kania-coaldust-6p25mgm3-28d |
|
| treated | MUC5AC, 12.5 mg/m3, 28 days GCM:kania-muc5ac-clsm-28d-output-12p5 · under EXPOSURE:kania-coaldust-12p5mgm3-28d |
|
| treated | MUC5AC, 25 mg/m3, 28 days GCM:kania-muc5ac-clsm-28d-output-25 · under EXPOSURE:kania-coaldust-25mgm3-28d |
Evidence
There was no significant difference in EGF and EGFR expressions for 28-d exposure (p>0.05), but there was a significant difference in MUC5AC expression (p<0.05).
States the significant MUC5AC result at 28 days that this assay records, together with the p<0.05 threshold reported for it.
We concluded that subchronic inhalation of coal dust particulate matter 10 induces bronchoalveolar reactive hyperplasia and rearrangement of epithelial cells which accompanied by decrease expression MUC5AC in male rats.
The paper's own conclusion gives the direction of the significant MUC5AC change as a decrease, which is why this assay informs on the decreased-MUC5AC key event.
Bronchoalveolar goblet cell hyperplasia by histology - coal dust PM10
Histological examination of the bronchoalveolar epithelium of male Wistar rats exposed to coal dust PM10 for 14 and 28 days. The paper describes epithelial rearrangement, hyperplastic (metaplastic) goblet cells and scattered massive inflammatory cells, and its conclusion calls this bronchoalveolar reactive hyperplasia. The finding is qualitative: no goblet cell counts, percentages or scores are reported in the available abstract.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Coal dust PM10, 25 mg/m3, 14 days EXPOSURE:kania-coaldust-25mgm3-14d |
coal dust ENVO:02000099 |
25 milligram per cubic meter | 14 day |
| Coal dust PM10, 25 mg/m3, 28 days EXPOSURE:kania-coaldust-25mgm3-28d |
coal dust ENVO:02000099 |
25 milligram per cubic meter | 28 day |
Outputs
| Group | Output | Description |
|---|---|---|
| treated | Bronchoalveolar epithelial histology, 14 days GCM:kania-goblet-histology-output-14d · under EXPOSURE:kania-coaldust-25mgm3-14d |
|
| treated | Bronchoalveolar epithelial histology, 28 days GCM:kania-goblet-histology-output-28d · under EXPOSURE:kania-coaldust-25mgm3-28d |
Evidence
The bronchoalveolar epithelial image of the group exposed to coal dust for 14 and 28 days showed a epithelial rearrangement, hyperplastic (metaplastic) goblet cells, and scattered massive inflammatory cells.
Records the goblet cell hyperplasia observed in coal-dust-exposed rats at both exposure durations, which is the key event this assay informs on.
The pulmonary parenchymal image of the group of exposed to coal dust for 14 and 28 days showed scattered inflammatory cells filling up the pulmonary alveolar networks, leading to an appearance of thickened parenchymal alveoli until emphysema-like structure.
Describes the accompanying parenchymal inflammation and alveolar thickening recorded in this assay's description, establishing the tissue context of the hyperplastic response.
EGFR signaling assays
EGFR and EGF expression - coal dust PM10, 14 days
EGFR expression measured by confocal laser scanning microscopy and EGF expression measured by ELISA in male Wistar rat lung after 14 days of coal dust PM10 inhalation at 6.25, 12.5 and 25 mg/m3. The paper reports no significant difference in either EGF or EGFR expression for the 14-day exposure (p>0.05). No measurement values, group sizes or exact p-values are reported in the available abstract.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Coal dust PM10, 6.25 mg/m3, 14 days EXPOSURE:kania-coaldust-6p25mgm3-14d |
coal dust ENVO:02000099 |
6.25 milligram per cubic meter | 14 day |
| Coal dust PM10, 12.5 mg/m3, 14 days EXPOSURE:kania-coaldust-12p5mgm3-14d |
coal dust ENVO:02000099 |
12.5 milligram per cubic meter | 14 day |
| Coal dust PM10, 25 mg/m3, 14 days EXPOSURE:kania-coaldust-25mgm3-14d |
coal dust ENVO:02000099 |
25 milligram per cubic meter | 14 day |
Outputs
| Group | Output | Description |
|---|---|---|
| treated | EGFR and EGF, 6.25 mg/m3, 14 days EGFR:kania-egfr-egf-14d-output-6p25 · under EXPOSURE:kania-coaldust-6p25mgm3-14d |
|
| treated | EGFR and EGF, 12.5 mg/m3, 14 days EGFR:kania-egfr-egf-14d-output-12p5 · under EXPOSURE:kania-coaldust-12p5mgm3-14d |
|
| treated | EGFR and EGF, 25 mg/m3, 14 days EGFR:kania-egfr-egf-14d-output-25 · under EXPOSURE:kania-coaldust-25mgm3-14d |
Evidence
There was no significant difference in MUC5AC, EGF, and EGFR expressions for 14-d exposure (p>0.05).
This is the paper's statistical result for EGF and EGFR at 14 days and is the null finding this assay records.
EGF expressions in rat lungs were measured by ELISA.
Names the technique used for the EGF ligand measurement this assay records, matching the ELISA protocol it follows.
EGFR and EGF expression - coal dust PM10, 28 days
EGFR expression measured by confocal laser scanning microscopy and EGF expression measured by ELISA in male Wistar rat lung after 28 days of coal dust PM10 inhalation at 6.25, 12.5 and 25 mg/m3. The paper reports no significant difference in either EGF or EGFR expression for the 28-day exposure (p>0.05), in contrast to the significant MUC5AC change at the same timepoint. No measurement values, group sizes or exact p-values are reported in the available abstract.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Coal dust PM10, 6.25 mg/m3, 28 days EXPOSURE:kania-coaldust-6p25mgm3-28d |
coal dust ENVO:02000099 |
6.25 milligram per cubic meter | 28 day |
| Coal dust PM10, 12.5 mg/m3, 28 days EXPOSURE:kania-coaldust-12p5mgm3-28d |
coal dust ENVO:02000099 |
12.5 milligram per cubic meter | 28 day |
| Coal dust PM10, 25 mg/m3, 28 days EXPOSURE:kania-coaldust-25mgm3-28d |
coal dust ENVO:02000099 |
25 milligram per cubic meter | 28 day |
Outputs
| Group | Output | Description |
|---|---|---|
| treated | EGFR and EGF, 6.25 mg/m3, 28 days EGFR:kania-egfr-egf-28d-output-6p25 · under EXPOSURE:kania-coaldust-6p25mgm3-28d |
|
| treated | EGFR and EGF, 12.5 mg/m3, 28 days EGFR:kania-egfr-egf-28d-output-12p5 · under EXPOSURE:kania-coaldust-12p5mgm3-28d |
|
| treated | EGFR and EGF, 25 mg/m3, 28 days EGFR:kania-egfr-egf-28d-output-25 · under EXPOSURE:kania-coaldust-25mgm3-28d |
Evidence
There was no significant difference in EGF and EGFR expressions for 28-d exposure (p>0.05), but there was a significant difference in MUC5AC expression (p<0.05).
States the null EGF and EGFR result at 28 days that this assay records, and separates it from the significant MUC5AC change at the same timepoint.
The purpose of the present study was to evaluate the effects of PM10 coal dust on lung histology, MUC5AC expression, epidermal growth factor (EGF) expression, and epidermal growth factor receptor (EGFR) expression.
Confirms that EGF and EGFR expression were prespecified endpoints of the study rather than incidental observations, which is what this assay's two measured targets represent.
Response comparisons
MUC5AC expression after 14-day coal dust exposure
Paper reports no significant difference (p>0.05) for MUC5AC at 14 days. No values, exact p-value or comparator group values are given in the available abstract, so only the direction is recorded.
MUC5AC expression after 28-day coal dust exposure
Paper reports a significant difference for MUC5AC at 28 days (p<0.05) and its conclusion states the direction is a decrease. No values, exact p-value or comparator group values are given in the available abstract, so change_value is omitted.
EGFR expression after 14-day coal dust exposure
Paper reports no significant difference (p>0.05) for EGFR at 14 days. No values or exact p-value are given in the available abstract.
EGFR expression after 28-day coal dust exposure
Paper reports no significant difference (p>0.05) for EGFR at 28 days. No values or exact p-value are given in the available abstract.
EGF expression after 14-day coal dust exposure
Paper reports no significant difference (p>0.05) for EGF at 14 days. No values or exact p-value are given in the available abstract.
EGF expression after 28-day coal dust exposure
Paper reports no significant difference (p>0.05) for EGF at 28 days. No values or exact p-value are given in the available abstract.
Protocols
Subchronic coal dust PM10 inhalation exposure
Subchronic inhalation exposure of male Wistar rats to coal dust particulate matter 10 at 6.25, 12.5 and 25 mg/m3 for 14 or 28 days. The abstract does not report chamber type, daily exposure hours, particle characterisation or group sizes.
Equipment: Coal dust PM10 inhalation exposure apparatus
ELISA for EGF in rat lung
Epidermal growth factor (EGF) expression in rat lungs measured by enzyme-linked immunosorbent assay. The abstract does not report kit manufacturer, dilutions or standard curve details.
Equipment: ELISA plate reader
Confocal laser scanning microscopy for EGFR and MUC5AC
EGFR and MUC5AC expression measured by a confocal laser scanning microscope on rat lung tissue. The abstract does not report antibodies, dilutions or the quantification method.
Equipment: Confocal laser scanning microscope
Lung histology of bronchoalveolar epithelium and pulmonary parenchyma
Histological examination of the bronchoalveolar epithelial and pulmonary parenchymal images of rat lung after 14 and 28 days of coal dust exposure. The abstract does not name the stain used or a scoring scheme.
Equipment: Light microscope