Effects of environmental air pollutants on CFTR expression and function in human airway epithelial cells.
Study subjects
Calu-3 human airway epithelial cells
CFTR assays
CFTR function - Calu-3, tobacco smoke extract 24 h
CFTR function in Calu-3 human airway epithelial cells after 24 h exposure to tobacco smoke extract. The abstract reports that tobacco smoke extract led to acquired CFTR dysfunction. No magnitude, concentration, sample size or p-value is reported in the abstract, so the output records carry no values.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Vehicle control, 24 hours (unexposed Calu-3) EXPOSURE:nguyen-vehicle-24h |
— | — | 24 hour |
| Tobacco smoke extract for 24 hours (concentration not reported in abstract) EXPOSURE:nguyen-tse-24h |
smoke ENVO:01000838 |
— | 24 hour |
Outputs
| Group | Output | Description |
|---|---|---|
| vehicle_control | Vehicle control CFTR function (no values reported in abstract) CFTR:nguyen-tse-24h-output-control · under EXPOSURE:nguyen-vehicle-24h |
|
| treated | Tobacco smoke extract CFTR function - dysfunction reported, no values in abstract CFTR:nguyen-tse-24h-output-tse · under EXPOSURE:nguyen-tse-24h |
Evidence
We demonstrate that both tobacco smoke extract and diesel exhaust particles led to acquired CFTR dysfunction
The abstract's stated result names tobacco smoke extract as one of the two pollutants that produced acquired CFTR dysfunction, which is the decreased CFTR function this assay's treated output records.
CFTR function - Calu-3, urban particulate matter 24 h
CFTR function in Calu-3 cells after 24 h exposure to urban particulate matter. Urban particulate matter was one of the three pollutants the study set out to test, but the abstract's result sentence names only tobacco smoke extract and diesel exhaust particles as causing acquired CFTR dysfunction. Recorded here as a tested exposure with REFUTE evidence against decreased CFTR function; the abstract reports no values.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Vehicle control, 24 hours (unexposed Calu-3) EXPOSURE:nguyen-vehicle-24h |
— | — | 24 hour |
| Urban particulate matter for 24 hours (concentration not reported in abstract) EXPOSURE:nguyen-upm-24h |
respirable suspended particulate matter ENVO:01000405 |
— | 24 hour |
Outputs
| Group | Output | Description |
|---|---|---|
| vehicle_control | Vehicle control CFTR function (no values reported in abstract) CFTR:nguyen-upm-24h-output-control · under EXPOSURE:nguyen-vehicle-24h |
|
| treated | Urban particulate matter CFTR function - no dysfunction reported, no values in abstract CFTR:nguyen-upm-24h-output-upm · under EXPOSURE:nguyen-upm-24h |
Evidence
The aim of our study was to test whether environmental air pollutants tobacco smoke extract, urban particulate matter, and diesel exhaust particles lead to acquired CFTR dysfunction
The abstract's aim sentence establishes that urban particulate matter was one of the three pollutant exposures actually tested against CFTR readouts, which is what this assay record's exposure condition represents.
We demonstrate that both tobacco smoke extract and diesel exhaust particles led to acquired CFTR dysfunction
The result sentence attributes acquired CFTR dysfunction to tobacco smoke extract and diesel exhaust particles only, so it argues against decreased CFTR function for the urban particulate matter arm.
CFTR function - Calu-3, diesel exhaust particles 24 h
CFTR function in Calu-3 cells after 24 h exposure to diesel exhaust particles. The abstract reports that diesel exhaust particles led to acquired CFTR dysfunction. No magnitude, concentration, sample size or p-value is reported in the abstract.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Vehicle control, 24 hours (unexposed Calu-3) EXPOSURE:nguyen-vehicle-24h |
— | — | 24 hour |
| Diesel exhaust particles for 24 hours (concentration not reported in abstract) EXPOSURE:nguyen-dep-24h |
diesel exhaust ENVO:03510008 |
— | 24 hour |
Outputs
| Group | Output | Description |
|---|---|---|
| vehicle_control | Vehicle control CFTR function (no values reported in abstract) CFTR:nguyen-dep-24h-output-control · under EXPOSURE:nguyen-vehicle-24h |
|
| treated | Diesel exhaust particle CFTR function - dysfunction reported, no values in abstract CFTR:nguyen-dep-24h-output-dep · under EXPOSURE:nguyen-dep-24h |
Evidence
We demonstrate that both tobacco smoke extract and diesel exhaust particles led to acquired CFTR dysfunction and that rescue of acquired CFTR dysfunction is possible with pharmacological interventions in diesel exhaust particle models.
The abstract's result sentence names diesel exhaust particles as a pollutant that produced acquired CFTR dysfunction, which is the decreased CFTR function this assay's treated output records.
CFTR function rescue - Calu-3, diesel exhaust particles plus pharmacological intervention, 24 h
CFTR function in Calu-3 cells co-treated with diesel exhaust particles and a pharmacological intervention for 24 h. The abstract reports that rescue of acquired CFTR dysfunction was possible with pharmacological interventions in the diesel exhaust particle models, but does not name the drug(s), the concentration, or the size of the rescue, so no agent term or value is asserted for the intervention.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Diesel exhaust particles for 24 hours (concentration not reported in abstract) EXPOSURE:nguyen-dep-24h |
diesel exhaust ENVO:03510008 |
— | 24 hour |
| Diesel exhaust particles plus pharmacological intervention for 24 hours (intervention not named in abstract) EXPOSURE:nguyen-dep-plus-drug-24h |
diesel exhaust ENVO:03510008 |
— | 24 hour |
Outputs
| Group | Output | Description |
|---|---|---|
| treated | Diesel exhaust particles alone, CFTR function (no values reported in abstract) CFTR:nguyen-dep-rescue-24h-output-dep · under EXPOSURE:nguyen-dep-24h |
|
| treated | Diesel exhaust particles plus pharmacological intervention, CFTR function rescued (no values in abstract) CFTR:nguyen-dep-rescue-24h-output-dep-drug · under EXPOSURE:nguyen-dep-plus-drug-24h |
Evidence
rescue of acquired CFTR dysfunction is possible with pharmacological interventions in diesel exhaust particle models
The abstract states directly that pharmacological intervention restored CFTR function in the diesel exhaust particle exposure, which is what this rescue arm records.
Gene expression assays
CFTR expression - Calu-3, tobacco smoke extract 24 h
CFTR protein expression readout in Calu-3 cells after 24 h tobacco smoke extract exposure. The abstract states CFTR expression was measured but does not report the result, so the output record carries no value.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Vehicle control, 24 hours (unexposed Calu-3) EXPOSURE:nguyen-vehicle-24h |
— | — | 24 hour |
| Tobacco smoke extract for 24 hours (concentration not reported in abstract) EXPOSURE:nguyen-tse-24h |
smoke ENVO:01000838 |
— | 24 hour |
Outputs
| Group | Output | Description |
|---|---|---|
| vehicle_control | Vehicle control CFTR expression (no values reported in abstract) GE:nguyen-cftr-expression-tse-24h-output-control · under EXPOSURE:nguyen-vehicle-24h |
|
| treated | Tobacco smoke extract CFTR expression (no values reported in abstract) GE:nguyen-cftr-expression-tse-24h-output-tse · under EXPOSURE:nguyen-tse-24h |
Evidence
Human airway epithelial cells (Calu-3) were exposed to air pollutant extracts for 24 h, with and without pharmacological interventions, with readouts of CFTR expression and function.
The methods sentence of the abstract establishes that CFTR expression was read out in Calu-3 cells after a 24 h air pollutant extract exposure, which is exactly what this assay record captures; the abstract reports no expression value, so none is recorded.
CFTR expression - Calu-3, urban particulate matter 24 h
CFTR protein expression readout in Calu-3 cells after 24 h urban particulate matter exposure. The abstract states CFTR expression was measured but does not report the result, so the output record carries no value.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Vehicle control, 24 hours (unexposed Calu-3) EXPOSURE:nguyen-vehicle-24h |
— | — | 24 hour |
| Urban particulate matter for 24 hours (concentration not reported in abstract) EXPOSURE:nguyen-upm-24h |
respirable suspended particulate matter ENVO:01000405 |
— | 24 hour |
Outputs
| Group | Output | Description |
|---|---|---|
| vehicle_control | Vehicle control CFTR expression (no values reported in abstract) GE:nguyen-cftr-expression-upm-24h-output-control · under EXPOSURE:nguyen-vehicle-24h |
|
| treated | Urban particulate matter CFTR expression (no values reported in abstract) GE:nguyen-cftr-expression-upm-24h-output-upm · under EXPOSURE:nguyen-upm-24h |
Evidence
with readouts of CFTR expression and function
The abstract names CFTR expression as one of the two readouts taken on the pollutant-exposed Calu-3 cells, which is what this urban particulate matter expression record documents; no expression value is reported, so none is recorded.
CFTR expression - Calu-3, diesel exhaust particles 24 h
CFTR protein expression readout in Calu-3 cells after 24 h diesel exhaust particle exposure. The abstract states CFTR expression was measured but does not report the result, so the output record carries no value.
Exposure conditions
| Condition | Agent | Concentration | Duration |
|---|---|---|---|
| Vehicle control, 24 hours (unexposed Calu-3) EXPOSURE:nguyen-vehicle-24h |
— | — | 24 hour |
| Diesel exhaust particles for 24 hours (concentration not reported in abstract) EXPOSURE:nguyen-dep-24h |
diesel exhaust ENVO:03510008 |
— | 24 hour |
Outputs
| Group | Output | Description |
|---|---|---|
| vehicle_control | Vehicle control CFTR expression (no values reported in abstract) GE:nguyen-cftr-expression-dep-24h-output-control · under EXPOSURE:nguyen-vehicle-24h |
|
| treated | Diesel exhaust particle CFTR expression (no values reported in abstract) GE:nguyen-cftr-expression-dep-24h-output-dep · under EXPOSURE:nguyen-dep-24h |
Evidence
exposed to air pollutant extracts for 24 h, with and without pharmacological interventions, with readouts of CFTR expression and function
This fragment of the abstract's methods sentence establishes the 24 h pollutant-extract exposure and the CFTR expression readout that this diesel exhaust particle record captures; the abstract reports no expression value.
Key-event relationships
Decreased CFTR function leads to impaired mucociliary clearance
Framing stated in the abstract's background: mucociliary clearance defends the airway against environmental air pollutants and is regulated by ion transporters including CFTR, so pollutant-acquired CFTR dysfunction acts on mucociliary clearance. Asserted as background in this paper, not measured in it.
Mucociliary clearance is an important host defense mechanism against environmental air pollutants and this process is regulated by various ion transporters including the cystic fibrosis transmembrane conductance regulator (CFTR).
The abstract states that mucociliary clearance is regulated by CFTR, which is the causal link from decreased CFTR function to impaired mucociliary clearance recorded here; the paper states it as background rather than measuring clearance, hence weak support.
Protocols
Calu-3 air pollutant extract exposure, 24 hours
Human airway epithelial cells (Calu-3) exposed to air pollutant extracts (tobacco smoke extract, urban particulate matter, or diesel exhaust particles) for 24 h, with and without pharmacological interventions. Extract preparation, concentrations and vehicle are not reported in the abstract, which is the only text available for this paper.
CFTR function readout in Calu-3 cells
Electrophysiological readout of CFTR function after 24 h pollutant extract exposure. The abstract states only that CFTR function was a readout; the cached record is indexed under the MeSH heading 'Membrane Potentials/drug effects'. Chamber type, stimulation agent, inhibitor and units are not reported in the abstract.
CFTR expression readout by immunoblot
CFTR protein expression measured after 24 h pollutant extract exposure. The abstract states only that CFTR expression was a readout; the cached record is indexed under the MeSH heading 'Blotting, Western'. Antibodies, loading control and quantification are not reported in the abstract.